SOLVED:P 17 G .6 The Förster theory of resonance energy transfer and the basis for the FRET technique can be tested by performing fluorescence measurements on a series of compounds in which an energy donor and an energy acceptor are covalently linked by a rigid molecular linker of variable and known length. L. Stryer and R.P. Haugland (Proc. Natl. Acad. Sci. USA 58,719(1967) ) collected the following data on energy transfer efficiencies, ηT, for a family of compounds with the general composition

Förster Resonance Energy Transfer (FRET) Microscopy