eLife

Broad-spectrum immune suppression encoded in self-amplifying RNA enables non-cytotoxic, non-immunostimulatory, externally controllable transgene expression

Differential effects of moderate and strong dsRNA-sensing pathway inhibition on saRNA transgene expression and cell loss. a, Schematic of the native saRNA, E3, and E3-NSs-L* constructs designed to ...
News Medical

Non-Invasive Particle Size Monitoring of Polymer-Based Vectors for Cell Transfection in Process Vessels

Accurate monitoring of nanoparticle size and growth is essential to ensure scalability in the development of transfection vectors for gene delivery. This study highlights how Spatially Resolved-DLS ...
EurekAlert!

Researchers use cholesterol-enriched exosomes to improve siRNA delivery

RNA interference (RNAi) technology has gradually become a cutting-edge technology for treating diseases such as genetic disorders and cancer due to its huge potential in gene expression regulation.
BioTechniques

Transfection: what it is and how to do it

Hinnah Campwala (left) is a Principal Scientist in the Cell Biology Development Group at Sartorius BioAnalytical Instruments (Michigan, USA). With a background in drug discovery and immune cell ...
The EMBO Journal

LINE-1 RNA triggers matrix formation in bone cells via a PKR-mediated inflammatory response

Peer ReviewDownload a summary of the editorial decision process including editorial decision letters, reviewer comments and author responses to feedback. Transposable elements (TEs) are mobile genetic ...
eLife

Co-expression of the AsCas12a ultra variant, a T7 RNA Polymerase and a cytosine base editor greatly increases transfection and editing rates in Leishmania species

Not revised: This Reviewed Preprint includes the authors’ original preprint (without revision), an eLife assessment, public reviews, and a response from the authors (if available). While CRISPR/Cas ...
Frontiers

Single cell phototransfection of mRNAs encoding SARS-CoV2 spike and nucleocapsid into human astrocytes results in RNA dependent translation interference

Multi-RNA co-transfection is starting to be employed to stimulate immune responses to SARS-CoV-2 viral infection. While there are good reasons to utilize such an approach, there is little background ...
Too Old to Operate

RNA-binding protein 14 promotes phase separation to sustain prostate specific antigen expression under androgen deprivation in human prostate cancer.

Castration-resistant prostate cancer (CRPC) is a big challenge in managing prostate cancer patients. The androgen receptor (AR) pathway is a major driver even in CRPC under androgen deprivation. The ...