Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, No. 26 (Dec. 23, 1997), pp. 14602-14607 (6 pages) The production of subtle or conditional mutations in mice ...

The Cre recombinase (Cre) from bacteriophage P1 is an important tool for genetic engineering in mammalian cells. We constructed lentiviral vectors that efficiently deliver Cre in vitro and in vivo.

Strategies for altering constitutional or somatic genotype in mice are well established, but approaches to generate mosaic genotypes in mouse tissues are limited. We showed that a functionally ...

New tools are now available for generating specifically targeted genetic mutations in bacteria, mammalian cells and mice. The new recombinase, Dre, is similar to its predecessor, Cre, but targets ...

Chances are if you use mice in your research, you are intimately acquainted with the Cre/LoxP recombination system. (For the uninitiated out there, this is a system to achieve tissue- or cell ...

Cre-loxP, a powerful tool for preclinical cancer research, possesses intrinsic pitfalls that can alter result accuracy, according to an editorial paper in Oncoscience. Cancer and other diseases could ...

Researchers design a Cas9 enzyme that cuts DNA only in the presence of particular drug. A series of rodent experiments showed that even with abundant food and water, personal space is essential to ...

A team of Chinese researchers has developed two new genome editing technologies, known collectively as Programmable Chromosome Engineering (PCE) systems. The study, published online in Cell on August ...

In traditional knockout studies, the gene is deleted altogether. However, deleting genes that are essential to embryonic development is lethal. Conditional gene knockout can be activated in an adult ...

Species: Mouse Genes: Gba1 Modification: Gba1: Conditional Knock-out Disease Relevance: Parkinson's Disease Strain Name: B6.129-Gba1 tm1Clk /J Genetic Background: Backcrossed 11 generations to ...

(Nanowerk News) A team of Chinese researchers led by Prof. GAO Caixia from the Institute of Genetics and Developmental Biology of the Chinese Academy of Sciences has developed two new genome editing ...