The ability to alter the genetic composition of living cells has revolutionized biology. Scientific advances, from treating genetic disorders through gene therapy to reprograming skin cells into ...

This protocol outlines steps for optimizing the transfection of adherent primary mammalian cells using the readily available off-the-shelf cationic polymer, 25-kDa branched polyethylenimine (bPEI25).

Striking the Right Balance Becomes More Challenging If Co-Transfection Scenarios Are Planned Transfection technology awaits no rallying cry. It hastens from siege to siege, attacking cellular bastions ...

Transfection, a key genetic technology in the toolbox of many researchers, spans numerous applications—production of recombinant proteins and recombinant cell lines, drug discovery, delivery of ...

The transfection of genes and the delivery of cell membrane–impermeable biomolecules—potentially drugs—into mammalian cells are among the most important research tools in modern molecular biology. A ...

Transfection alters the genetic makeup of eukaryotic cells by introducing foreign nucleic acids, including DNA, RNA, and small noncoding RNAs such as siRNA, shRNA, and miRNA. Scientists use ...

Hinnah Campwala (left) is a Principal Scientist in the Cell Biology Development Group at Sartorius BioAnalytical Instruments (Michigan, USA). With a background in drug discovery and immune cell ...