Regeneron is integrating single-use chromatographic clarification into its commercial-scale process for manufacturing adeno-associated vectors (AAVs), which enables it to eliminate endonuclease in ...

Reconstitution of the activation of the MLH1–MLH3 endonuclease shows how crossovers are formed during meiosis. When DNA breaks, cells use the homologous recombination pathway to accurately repair the ...

Apurinic/apyrimidinic endonuclease 1 (APE1), also known as Ref-1, performs critical dual functions as both a DNA repair enzyme and a redox signalling regulator. Within the base excision repair pathway ...

Homologous recombination is a DNA repair mechanism that counteracts double-stranded breaks in DNA. Researchers at Kindai University have recently revealed how the Sae2 protein coordinates with the ...

Effects of setup margin and biology tracking zone variations on dosimetric plan quality and treatment feasibility in biology-guided radiotherapy for bone metastasis.

Flap endonuclease 1 (FEN1) is an essential DNA repair enzyme whose roles in replication and genomic maintenance have made it a critical target in cancer research. Elevated levels of FEN1 have been ...

Molecular cloning relies on two key components to construct recombinant DNA: 1) a DNA vector and 2) one or more DNA fragments containing the gene(s) of interest (GOI). To produce a GOI fragment, DNA ...

Scientists first observed the biological phenomenon of restriction and modification in the 1950s, but they did not identify and isolate restriction enzymes until the late 1960s. 3 In 1970, Hamilton ...