AcrIIA15 is an anti-CRISPR (Acr) protein that inhibits Staphylococcus aureus Cas9 (SaCas9). Although previous studies suggested it has dual functions, the structural and biochemical basis for its two ...
The overall binding of the Aca2 dimer to dsRNA resembles its DNA binding 18, but with key differences (Fig. 4b). Insertion of the HTH domains into the dsRNA grooves distorts the protein compared with ...
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