The use of a PRG allows us to monitor Cre recombinase-mediated recombination noninvasively and repeatedly in the same animal. In addition, this approach may also allow us to track the in vivo ...

Here we describe the generation of a double-fluorescent Cre/loxP indicator system. This protocol involves (i) all cloning steps to generate the plasmid vector (3–5 months); (ii) a guide to prepare ...

Mice and newts were applied to the one-step Cre-loxP organism creation technology by TAx9. In this study, the researchers succeeded in synthesizing a Cre-loxP integrated vector by placing a short DNA ...

A new editorial paper was published in Oncoscience (Volume 10) on November 14, 2023, entitled, "Be mindful of potential pitfalls when using the Cre-LoxP system in cancer research." In this new ...

In traditional knockout studies, the gene is deleted altogether. However, deleting genes that are essential to embryonic development is lethal. Conditional gene knockout can be activated in an adult ...

The research was led by Professor Wang Lixin (Department of Vascular Surgery, Zhongshan Hospital, Fudan University) and Professor Zhou Bin (Shanghai Institute of Biochemistry and Cell Biology, Center ...

Chances are if you use mice in your research, you are intimately acquainted with the Cre/LoxP recombination system. (For the uninitiated out there, this is a system to achieve tissue- or cell ...

All our multiexpression systems (currently MultiBac, MultiMam and MultiColi) rely on in vitro Cre-loxP recombination of Acceptor and Donor plasmids to assemble multiprotein expression vectors. A ...

Researchers design a Cas9 enzyme that cuts DNA only in the presence of particular drug. A series of rodent experiments showed that even with abundant food and water, personal space is essential to ...

We have created a stable transgenic rag2-EGFP-mMyc zebrafish line that develops GFP-labeled T cell acute lymphoblastic leukemia (T-ALL), allowing visualization of the onset and spread of this disease.